منابع مشابه
The Molecular Weight of Crystalline Catalase.
In 1933 Stern (2) used the diffusion method of Northrop and Anson (3) to determine the molecular weights of purified catalase from horse liver, pig liver, cow liver, horse kidney, and cow erythrocytes. He found the average value for the molecular weight of catalase to be 68,900, and stated that the catalase molecule has a dimension similar to that of hemoglobin. In 1938 Stern and Wyckoff (4) pu...
متن کاملPreparation of crystalline erythrocuprein and catalase from human erythrocytes.
The removal of hemoglobin from blood cell lysates used for the preparation of various erythrocyte enzymes has been commonly achieved by denaturation through the use of a chloroform-ethanol mixture, the so-called Tsuchihashi procedure (1). Although there has been speculation concerning possible modification of the nonhemoglobin protein by such treatment (2, 3), no clear cut, alternative, large s...
متن کاملPreparation of Crystalline Erythocuprein and Catalase from Human Erythrocytes*
The removal of hemoglobin from blood cell lysates used for the preparation of various erythrocyte enzymes has been commonly achieved by denaturation through the use of a chloroform-ethanol mixture, the so-called Tsuchihashi procedure (1). Although there has been speculation concerning possible modification of the nonhemoglobin protein by such treatment (2, 3), no clear cut, alternative, large s...
متن کاملA solubility analysis of crystalline ox-liver catalase.
Sedimentation. This was observed in a Svedberg oilturbine ultracentrifuge by the method of Cecil & Ogston (1948). The hydrochlorides were dissolved in 0-2M-NaCI to a concentration of 1 g./100 ml. Owing to the relatively small molecular weights and high diffusion coefficients, clear sedimentation boundaries were not obtained; the sedimentation constants were therefore measured bythe method ofGut...
متن کاملIs Root Catalase a Bifunctional Catalase-Peroxidase?
Plant catalases exhibit spatial and temporal distribution of their activity. Moreover, except from the typical monofunctional catalase, a bifunctional catalase-peroxidase has been reported. The aim of this study was to investigate whether the leaf and root catalases from six different plant species (Lactuca sativa, Cichorium endivia, Apium graveolens, Petroselinum crispum, Lycopersicon esculent...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1937
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)74274-9